Paper Key : IRJ************052
Author: Thatha Kuntla Meghana,Dr. M. Ajitha
Date Published: 02 Nov 2024
Abstract
An HPLC method was developed to simultaneously quantify Pregabalin and Etoricoxib in tablet dosage forms. A Kromasil column was used, with a mobile phase of 0.1% Perchloric Acid and Acetonitrile in a 40:60 ratio. The flow rate and temperature were set at 1 mLmin and 30C, respectively, with detection at 230 nm. The retention times for Pregabalin and Etoricoxib were 2.463 and 2.815 minutes. Regression equations showed high linearity for both drugs (Etoricoxib: y 19095x + 21003; Pregabalin: y 19173x + 28445). %RSD values were 0.3% for Pregabalin and 0.4% for Etoricoxib, indicating good accuracy. Recoveries confirmed the method's accuracy at 99.14% for Pregabalin and 99.95% for Etoricoxib. The limits of detection (LOD) for Pregabalin were 0.32, and the quantification limit (LOQ) was 0.97; for Etoricoxib, the LOD was 0.30, and the LOQ was 0.91. This method is effective for routine quality control analysis of both drugs in the pharmaceutical industry, offering shorter retention and run times.