Paper Key : IRJ************216
Author: Anusha B
Date Published: 27 Oct 2023
Abstract
ABSTRACT Bromophenol blue can cause irritation with redness and pain when it comes in contact with the skin; can causes irritation to the respiratory tract and leads to allergic reaction. Symptoms may include coughing and shortness of breath. Natural pigment anthocyanin was extracted from the samples clitorea ternatea and tecoma stans by normal centrifugation process. So, this study is aimed to find alternative tracking dye for natural sources to replace the synthetic Bromophenol blue dye. Natural pigment derived from the plant sources including chlorophyll, carotenoids, flavonoids, anthocyanin, quinones, betacyanin etc. Natural dye have some similar characters to Bromophenol blue In this project I reported the stability of anthocyanin pigment at different pH temperature and demonstrated the effectiveness of pigment in tracking dye in agarose gel electrophoresis. That has content maintain their color over a wide range of pH 3 to 7. This flower sample is used as the tracking dye in place of Bromophenol blue. Every flower and leaves contain pigments but among them only some will have capacity to act as effective dye. Since, anthocyanin pigments are similar to Bromophenol blue, anthocyanin rich flowers like clitorea ternatea are used for the experiment. KEYWORDS: Clitorea ternatea, Tecoma stans, Tracking dye, Bromophenol blue, Gel electrophoresis, Natural pigments.
DOI LINK : 10.56726/IRJMETS45519 https://www.doi.org/10.56726/IRJMETS45519